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Protein Biochemistry
Proteomics Tools
Protein Purification and Sample Prep
EpiQuik Nuclear Extraction Kit
Supplier Images
EpiQuik Nuclear Extraction Kit
EpiQuik Nuclear Extraction Kit
EpiQuik Nuclear Extraction Kit from EpiGentek
Be the first to write a review!
Citations:
(179)
Supplier Page
Supplier Page from
EpiGentek for
EpiQuik Nuclear Extraction Kit
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
EpiQuik Nuclear Extraction Kit
Company
EpiGentek
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
OP-0002
Quantity
100 Assays
Applications
Sample Isolation
Format
Tube-based
Sample Type
Unisolated Samples
Research Area
Chromatin & Transcription, DNA Damage & Repair, DNA Methylation, Gene Expression & Silencing, Phosphorylation
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EpiGentek
110 Bi County Blvd. Ste 122
Farmingdale
,
NY
11735
United States
Phone
: 1 877 EPIGENTEK (374-4368)
International: 1 631 755-0888
Fax (General): 1 631 815-6729
Fax (Orders): 1 718 484-3956
Email: info@epigentek.com
sales@epigentek.com
services@epigentek.com
support@epigentek.com
Company Profile
Website:
www.epigentek.com
Citations (179)
(21)
Hydrogen Promotes the M1 Macrophage Conversion During the Polarization of Macrophages in Necrotizing Enterocolitis
Frontiers in pediatrics
January 1, 2021
Yu S, Lv Z, Gao Z, Shi J, Sheng Q, Zheng L, Zhou J, Wang X.
proteins. Cytoplasmic and nuclear proteins were extracted using the EpiQuickTM Nuclear Extraction Kit I (
OP-0002
;
EpiGentek
, Farmingdale, NYUSA) (32). The nucleoprotein concentration was quantified with bicinchoninic
(22)
Icariin enhances AMP-activated protein kinase and prevents high fructose and high salt-induced metabolic syndrome in rats
Saudi Pharmaceutical Journal
November 1, 2020
Abeer A. Aljehani, Nawal A. Albadr, Basma G. Eid, Ashraf B. Abdel-Naim
on the collected supernatants. 2.8 Nuclear extract preparation A
EpiQuik™ nuclear extraction kit
(
OP-0002
,
Epigentek
, NY, USA) was used for the preparation of nuclear extracts from liver samples. Levels of
(23)
The angiocrine Rspondin3 instructs interstitial macrophage transition via metabolic-epigenetic reprogramming and resolves inflammatory injury
Nature immunology
November 1, 2020
Zhou B, Magana L, Hong Z, Huang LS, Chakraborty S, Tsukasaki Y, Huang C, Wang L, Di A, Ganesh B,
et al
.
nuclear extracts were prepared from
macrophages
with
EpiQuik Nuclear Extraction Kit
(
Epigentek
, Cat#
OP-0002
), then 5 μg of nuclear protein from each sample were used to incubate with the TET or JMJD3 demethylase
(24)
التأثیر الوقائی للموز والزبادی على تقرحات المعدة فی الفئران The protective effect of banana and yoghurt on gastric ulceration in rats
مجلة البحوث فی مجالات التربیة النوعیة
November 1, 2020
عبور عبد الرحمن, الشیماء حافظ
homogenates were used to prepare nuclear extracts to determine TNF-α, using
EpiQuik™ nuclear extraction kit
(
OP-0002
,
EpiGentek
, NY, USA) according to Millena et al,. (2004).p-Nitrophenyl sulfite was found to be a suitable
(25)
Regulation of a long noncoding RNA MALAT1 by Aryl Hydrocarbon Receptor in pancreatic cancer cells and tissues.
Biochemical and Biophysical Research Communications
November 1, 2020
Ji-eun Lee, Sung-Gook Cho, Seong-Gyu Ko, Syed A. Ahrmad, Alvaro Puga, Kyounghyun Kim
extract from cells or pancreatic tissues were collected using
EpiQuik Nuclear Extraction kit
(Cat #
OP-0002
-1,
EpiGentek
, Inc., Farmingdale, NY). By using obtained nuclear extracts, we performed H3K27me3
enzymatic assay
(26)
Siva‑1 regulates multidrug resistance of gastric cancer by targeting MDR1 and MRP1 via the NF‑κB pathway
Molecular Medicine Reports
June 3, 2020
Fan‑Biao Kong, Qiao‑Ming Deng, Hong‑Qiang Deng, Chen‑Cheng Dong, Lei Li, Chun‑Gang He, Xiao‑Tong Wang, Sheng Xu, Wei Mai
manufacturer's protocol. Nuclear proteins were extracted using a
EpiQuik™ Nuclear Extraction kit
(cat. no.
OP-0002
;
EpiGentek
Group, Inc.), 1×107 cells were transferred to a 1.5-ml microcentrifuge tube and centrifuged
(27)
Study the effect of natural antioxidants in mulberry on prostate cancer in male Wister albino
مجلة الاقتصاد المنزلی
June 1, 2020
Abor M. M. Abd EL- Rahman, Amina Mohamed Amin Tolba
prepare nuclear extracts for the de termination of TNFα, using the nuclear extraction device EpiQuikTM (
OP0002
,
EpiGentek
, NY, US). Cells of
prostate cancer
(PC3) were grown to approximat ely 80 percent confluence
(28)
MYC Regulation of D2HGDH and L2HGDH Influences the Epigenome and Epitranscriptome
Cell chemical biology
May 21, 2020
Qiu Z, Lin AP, Jiang S, Elkashef SM, Myers J, Srikantan S, Sasi B, Cao JZ, Godley LA, Rakheja D,
et al
.
Methylation Quantification Kit,
Epigentek
,Cat#
P-9005
EpiQuik Nuclear Extraction Kit
I,
Epigentek
,Cat#
OP-0002
Gentra Puregene DNA purification kit,
Qiagen
,Cat#
158667
Luciferase
Assay
Systems,
Promega
,Cat#
E1500
β-Galactosidase
(29)
PRMT5 regulates T cell interferon response and is a target for acute graft-versus-host disease
JCI Insight
April 23, 2020
Katiri J. Snyder et al.
Reactions contained 20 μg of the indicated nuclear extracts (prepared with Nuclear Extraction Kit,
OP-0002
from
EpiGentek
) and were incubated for 120 minutes at 37°C. Absorbance (450 nm with a reference wavelength
(30)
Sex differences in hypothalamic-pituitary-adrenal axis regulation after chronic unpredictable stress
Brain and behavior
April 1, 2020
Palumbo MC, Dominguez S, Dong H.
separate lysates into nuclear and cytoplasmic fractions using a nuclear extraction kit (
Epigentek
,
OP‐0002
‐1) following kit protocol. Briefly, tissue was homogenized in 200 μL of NE1 and then allowed to incubate
(31)
The novel reversible LSD1 inhibitor SP-2577 promotes anti-tumor immunity in SWItch/Sucrose-NonFermentable (SWI/SNF) complex mutated ovarian cancer
PloS one
January 1, 2020
Soldi R, Ghosh Halder T, Weston A, Thode T, Drenner K, Lewis R, Kaadige MR, Srivastava S, Daniel Ampanattu S, Rodriguez Del Villar R,
et al
.
harvested daily and lysates were produced with
EpiQuik Nuclear Extraction Kit
(
EpiGentek
, catalog no.
OP-0002
-1). Nuclear proteins were resolved by
SDS-PAGE
and immunoblotted for BRG1/ SMARCA4 expression (Abcam,
(32)
Neonatal Inhibition of DNA Methylation Disrupts Testosterone-Dependent Masculinization of Neurochemical Phenotype
Endocrinology
January 1, 2020
Carla D Cisternas, Laura R Cortes, Ilona Golynker, Alexandra Castillo-Ruiz, Nancy G Forger
Nuclear protein was purified using the
EpiQuik Nuclear Extraction Kit
1 (
Epigentek
, Farmingdale, NY;
OP-0002
) and quantified by BCA
Protein Assay
(
Thermo Scientific
;
23252
). Total DNMT activity was evaluated using the
(33)
The DNMT1/miR-34a Axis Is Involved in the Stemness of Human Osteosarcoma Cells and Derived Stem-Like Cells
Stem Cells International
October 31, 2019
Xiao Liang, Chang Xu, Wanchun Wang, Xiang Li
OSLCs (1 × 106 ) or U2OS cells (1 × 106 ) were obtained by the
EpiQuik™ Nuclear Extraction Kit
(cat.
OP-0002
-1;
EpiGentek
Group Inc., Farmingdale, NY, USA). DNMT1 activity is nonradioactively measured by using
(34)
Hydrogen sulphide mitigates homocysteine-induced apoptosis and matrix remodelling in mesangial cells through Akt/FOXO1 signalling cascade
Cellular signalling
September 1, 2019
Majumder S, Ren L, Pushpakumar S, Sen U.
were from Sigma-Aldrich (St. Louis, MO) or available highest grade.
Epiquik nuclear extraction kit
(
OP-0002
–1), Direct-zol RNA miniprep (R2050S) were from
Epigentek
(Farmingdale, NY). EasyScript cDNA synthesis
(35)
The Inhibitory Effect of (−)-Epigallocatechin-3-Gallate on Breast Cancer Progression via Reducing SCUBE2 Methylation and DNMT Activity
Molecules
August 9, 2019
Jie Sheng, Weilin Shi, Hui Guo, Wenlin Long, Yuxin Wang, Jiangfa Qi, Jinbiao Liu, Yao Xu
5-aza-dC for 48 h, the cell nuclear extracts were isolated using
EpiQuik Nuclear Extraction Kit
(cat. no.
OP-0002
-1,
EpiGentek
, NY, USA) according to the manufacturer’s instructions. Total DNMT activity in nuclear
(36)
Tat expression led to increased histone 3 tri-methylation at lysine 27 and contributed to HIV latency in astrocytes through regulation of MeCP2 and Ezh2 expression
Journal of NeuroVirology
August 1, 2019
Ying Liu, Yinghua Niu, Lu Li, Khalid A. Timani, Victor L. He, Chris Sanburns, Jiafeng Xie, Johnny J. He
obtained following first treatment with hypotonic buffer, then with an
EpiQuik Nuclear Extraction Kit
(#
OP-0002
,
Epigentek
, Farmingdale, NY). An equal amount of nuclear lysate was used to determine the histone methyltransferase
(37)
Negative regulation of miR‐1275 by H3K27me3 is critical for glial induction of glioblastoma cells
Molecular Oncology
July 1, 2019
Jialuo Mai et al.
the instructions provided in, nuclear extracts were prepared using
EpiQuik™ Nuclear Extraction Kit
(
OP‐0002
;
Epigentek
, Farmingdale, NY, USA). We used an EpiQuik™ histone methyltransferase activity/inhibition
(38)
JAK2 regulates mismatch repair protein‐mediated epigenetic alterations in response to oxidative damage
Environmental and Molecular Mutagenesis
May 1, 2019
Ning Ding, Sam A. Miller, Sudha S. Savant, Heather M. O'Hagan
histone methyltransferase activity ELISA assay according to the manufacturer’s protocol (
EpiGenTEK
,
OP-0002
-1 and
P-3005
-48).
qPCR
(quantitative
Polymerase Chain Reaction
) Total RNA was first isolated (
Qiagen
,
(39)
Prebiotic reduction of brain histone deacetylase (HDAC) activity and olanzapine-mediated weight gain in rats, are acetate independent
Neuropharmacology
May 1, 2019
Amy Chia-Ching Kao, Ka Wai Chan, Daniel C. Anthony, Belinda R. Lennox, Philip WJ. Burnet
combined cortex/hippocampal tissue using the EpiQuikTM Nuclear Extraction Kit I (
Epigentek
, Cat. No.
OP-0002
), according to manufacturer’s instructions. Briefly, the tissue was gently homogenised with steel beads
(40)
Altered DNA Methylation in the Developing Brains of Rats Genetically Prone to High versus Low Anxiety
The Journal of Neuroscience
April 17, 2019
Chelsea R. McCoy et al.
One set of samples was used to isolate protein (
EpiQuik Nuclear Extraction kit
,
Epigentek
, catalog #
OP-0002
–1; n ⫽ 8/group). Extracted protein concentration was quantified using a BCA
protein assay
(Thermo Fisher Scientific),
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EpiQuik Nuclear Extraction Kit from EpiGentek
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